Flow Cytometry Core

Instrumentation: See next section for the list of the instruments and specifications. The FCM core has developed user registration protocols and standard operation procedures for each instrument. The process will be initiated by contacting the FCM core by an e-mail.

Cellular sorting (semi self-operated sorting): The Core provides the set-up for the user's particular cell sorting experiment in every session.

Flow cytometry consultation: Various aspects of flow cytometry including designing experiment protocols, sample preparation methods, assessing the project feasibility, interpretation of the flow cytometry data and preparation of data presentation.

• Recommended 20-min introduction for flow cytometry beginners: BD “Introduction to Flow Cytometry”
• Applications include cell cycle analysis (1D or 2D), assessment of apoptosis with various probes, cell number and viability assessment, dye dilution proliferation assays (such as CFSE dilution), multiparameter immunophenotyping (staining of cell surface and intracellular proteins), assessment of phosphorylation status of signaling proteins, cell functional studies using fluorescent probes (such as Calcium flux), multiplex bead assay and measuring proximity by fluorescence resonance energy transfer (FRET)

Work with us to use the following equipment for your research:

• BD Accuri C6 (two-laser, four-color) – two units
• Guava EasyCyte 8HT (two-laser, four-color) 
• BD FACS LSRFortessa SORP (five-laser, 21-color, high-performance model with HTS)
• BD FACSJazz (three-laser, six-color, installed in a biosafety cabinet) 
• MACS magnetic bead separator (manual)
• MACS tissue dissociator
• EVOS FL Inverted fluorescence microscope – for sorter users to observe the pre- and post-sorted cells.
• Cell culture facilities (Sanford Center room 1261) – for sorter users, CO2 incubator, low-speed centrifuge with sealed buckets, an inverted microscope, work BSC, refrigerator/freezer, sink.
• Workstations and software – two workstations, installed with FlowJo, FCSExpress, CFlow, MS Office, Guava Soft and Modfit LT, are accessible with or without network connection.

Use of Antimetastatic SOD3-Mimetic Albumin as a Primer in Triple Negative Breast Cancer. Shanta M. Messerli, Amanda M. Schaefer, Yongxian Zhuang, Bohdan J. Soltys, Noah Keime, Jenny Jin, Li Ma, Carleton J. C. Hsia, and W. Keith Miskimins. Journal of Oncology. Volume 2019, Article ID 3253696.

Propranolol Promotes Glucose Dependence and Synergizes with Dichloroacetate for Anti-Cancer Activity in HNSCC. Christopher T. Lucido, W. Keith Miskimins, and Paola D. Vermeer. Cancers (Basel). 2018 Dec; 10(12): 476.

Self-Transducible Bimodal PDX1-FOXP3 Protein Lifts Insulin Secretion and Curbs Autoimmunity, Boosting Tregs in Type 1 Diabetic Mice. Christina Amatya, Ilian A. Radichev, Jacob Ellefson, Mark Williams, and Alexei Y. Savinov. Mol Ther. 2018 Jan 3; 26(1): 184–198.

β2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC. Christopher T. Lucido, Juan L. Callejas-Valera, Paul L. Colbert, Daniel W. Vermeer, W. Keith Miskimins,William C. Spanos, and Paola D. Vermeer. Oncogenesis. 2018 Oct; 7(10): 81.

Genetic Ataxi Telangiectasia porcine model phenohttpcopies the multisystemic features of the human disease. Rosanna Beraldi, David K. Meyerholz, Alexei Savinov, Attila D. Kovács, Jill M. Weimer, Jordan A. Dykstra,Ryan D. Geraets, and David A. Pearce Biochim Biophys Acta. 2017 Nov; 1863(11): 2862–2870.

Loss of peripheral protection in pancreatic islets by proteolysis-driven impairment of VTCN1 (B7-H4) presentation is associated with the development of autoimmune diabetes. Ilian A. Radichev, Lilia V. Maneva-Radicheva, Christina Amatya, Maryam Salehi, Camille Parker, Jacob Ellefson, Paul Burn, and Alexei Y. Savinov. J Immunol. 2016 Feb 15; 196(4): 1495–1506.

Nardilysin-Dependent Proteolysis of Cell-Associated VTCN1 (B7-H4) Marks Type 1 Diabetes Development. Ilian A. Radichev, Lilia V. Maneva-Radicheva, Christina Amatya, Camille Parker, Jacob Ellefson, Clive Wasserfall, Mark Atkinson, Paul Burn and Alexei Y. Savinov. Diabetes 2014 Oct; 63(10): 3470-3482

Autoimmune Diabetes Is Suppressed by Treatment with Recombinant Human Tissue Kallikrein-1. Lilia Maneva-Radicheva, Christina Amatya, Camille Parker, Jacob Ellefson, Ilian Radichev, Arvind Raghavan, Matthew L. Charles, Mark S. Williams, Mark S. Robbins, and Alexei Y. Savinov. PLoS One. 2014; 9(9): e107213.

Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of HPV+ cancer. Daniel W. Vermeer, William C. Spanos, Paola D. Vermeer, Annie M. Bruns, Kimberly M. Lee, and John H. Lee. Int J Cancer. 2013 Jul; 133(1): 120–129.

The Type 1 Diabetes-Resistance locus Idd22 Controls Trafficking of Autoreactive Cytotoxic T Lymphocytes into the Pancreatic Islets of NOD Mice. Robert L. Whitener, Lisa Gallo Knight, Jianwei Li, Sarah Knapp, Shuyao Zhang, Mani Annamalai, Vadim M Pliner, Dongtao Fu, Ilian Radichev, Christina Amatya, Alexei Savinov, Arif Yurdagul, Jr, Shuai Yuan, John Glawe, Christopher G. Kevil, Jing Chen, Scott E. Stimpson, and Clayton E Mathews. J Immunol. 2017 Dec 15; 199(12): 3991–4000.

See our guide for more information on working with the Flow Cytometry Core, including:

• Instruments and equipment
• Samples that can be analyzed and sorted
• User registration prerequisites
• Research acknowledgements
• Biohazards and other hazards
• Experiment examples
• Facility oversight and contact information
• Hours of operation